Brown Institute for Brain Science

Two Trial Social Memory Test

Rationale of Test

The Two-Trial Social Memory test assesses cognition, namely the ability to recognize novel versus familiar animals, in rodent models of CNS disorders. Over the course of multiple exposures, rodents will become habituated to an intruder and no longer find them as interesting as a completely novel intruder. This test is useful in identifying social amnesia in transgenic mice exhibiting autistic symptoms or other social interaction deficits, as well as in identifying novel chemical entities affecting social memory.

Apparatus

Procedure

A never-before-met conspecific is placed into the home cage of a test animal for 5 minutes, and then removed. After a period of time, the same female is placed back in the home cage together with a novel, never-before-met conspecific for 5 minutes. All test trials are videotaped and subsequently analyzed for total body investigation, anogenital investigation, perioral investigation, body investigation, grooming behavior, sexual interaction, and otherwise undefined interaction.

Interpretation

Relevant Controls

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